Quantification of acetylated tubulin
نویسندگان
چکیده
منابع مشابه
Distribution of acetylated alpha-tubulin in Physarum polycephalum
The expression and cytological distribution of acetylated alpha-tubulin was investigated in Physarum polycephalum. A monoclonal antibody specific for acetylated alpha-tubulin, 6-11B-1 (Piperno, G., and M. T. Fuller, 1985, J. Cell Biol., 101:2085-2094), was used to screen for this protein during three different stages of the Physarum life cycle--the amoeba, the flagellate, and the plasmodium. We...
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At its most fundamental level, touch sensation requires the translation of mechanical energy into mechanosensitive ion channel opening, thereby generating electro-chemical signals. Our understanding of this process, especially how the cytoskeleton influences it, remains unknown. Here we demonstrate that mice lacking the α-tubulin acetyltransferase Atat1 in sensory neurons display profound defic...
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The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy after double-staining by 6-11B-1 and B-5-1-2, a monoclonal antibody specific for all alph...
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The distribution of acetylated ct-tubulin in rat cerebellum was examined and compared with that of total ct-tubulin and tyrosinated ¢t-tubulin. From immunoperoxidase-stained vibratome sections of rat cerebellum it was found that acetylated a-tubulin, detectable with monoclonal 6-11B-l, was preferentially enriched in axons compared with dendrites. Parallel fiber axons, in particular, were labele...
متن کاملComposite microtubules of the axon: quantitative analysis of tyrosinated and acetylated tubulin along individual axonal microtubules.
We have shown previously, using immunoelectron microscopy, that axonal microtubules (MTs) are composite, consisting of distinct domains that differ in their content of tyrosinated alpha-tubulin (tyr-tubulin). Here, we extend these studies using a novel preparation that permits visualization of individual axonal MTs over distances of several tens of micrometers using conventional immunofluoresce...
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ژورنال
عنوان ژورنال: Cytoskeleton
سال: 2013
ISSN: 1949-3584
DOI: 10.1002/cm.21111